Cholerae was performed as described by Hoshino et al.18 PCR amplification of an exogenous internal positive control incorporated into each sample and amplification of the 16S ribosomal RNA gene were used to control for PCR inhibitors and bacterial DNA, respectively. Statistical Analysis In the principal analysis, we assessed the protection conferred by two completely ingested doses of vaccine against cholera confirmed by rapid diagnostic testing.Sensitivity Analysis In the sensitivity analysis, we assessed the robustness of our study benefits by investigating associations separately in each study center and according to kidney function and the timing of biopsies. First, at both centers, individuals with complement-binding donor-particular anti-HLA antibodies had the cheapest rate of graft survival . Second, complement-binding donor-specific anti-HLA antibodies after transplantation remained individually connected with graft loss whether they had been detected in specimens from protocol-specified biopsies performed at 1 year or in specimens from biopsies performed during acute rejection in the first year . Furthermore, the addition of an acute-rejection variable to the ultimate multivariate model didn’t change the significant predictors of graft loss.